Protein:TPH2 |
Protein Summary |
 Gene summary |
| Gene name: TPH2 | ASpdb.0 ID: 121278 | Gene | Gene symbol | TPH2 | Gene ID | 121278 |
| Gene name | tryptophan hydroxylase 2 |
| Synonyms | ADHD7|NTPH |
| Cytomap | 12q21.1 |
| Type of gene | protein-coding |
| Description | tryptophan 5-hydroxylase 2neuronal tryptophan hydroxylasetryptophan 5-monooxygenase 2 |
| Modification date | 20240403 |
| UniProtAcc | Q8IWU9 |
| Gene ontology of this gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Gene | TPH2 | GO:0004510 | tryptophan 5-monooxygenase activity | 37608910 |
AS Summary |
| Information of the canonical protein with experimentally identified structure from PDB (2023). |
| UniProt Acc | File name | PDB ID | Method | Resolution | Chain | Start | End |
| Q8IWU9-1 | Q8IWU9-1_4v06_A.pdb | 4V06 | X-ray | 2.63 | A | 148 | 490 |
| ASpdb's canonical and alternatively spliced isoform information. |
| accession_id | gene_name | canonical_id | alternative_id | canonical_length | alternative_length | canonical_start | canonical_end | type | originalSEQ | variationSEQ | alternative_start | alternative_end |
| Q8IWU9 | TPH2 | Q8IWU9-1 | Q8IWU9-2 | 490 | 492 | 147 | 147 | Substitution | E | GKE | 147 | 149 |
| Multiple sequence alignment of our canonical and alternatively spliced TPH2 |
| Matched gene isoform IDs with Ensembl and RefSeq of our canonical and alternative spliced genes of TPH2 |
| UniProt-id | ENSG | ENST | ENSP |
| Q8IWU9-1 | ENSG00000139287.13 | ENST00000333850.4 | ENSP00000329093.3 |
| UniProt-id | NM ID | NP ID |
| Q8IWU9-1 | NM_173353.3 | NP_775489.2 |
| Amino acid sequences of our canonical and alternatively spliced TPH2 |
| accession_id | Protein sequence |
| Q8IWU9-1 | MQPAMMMFSSKYWARRGFSLDSAVPEEHQLLGSSTLNKPNSGKNDDKGNKGSSKREAATESGKTAVVFSLKNEVGGLVKALRLFQEKRVN MVHIESRKSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPENIWTEEEELEDVPWFPRKISELDKCSHRVLMYGSELDADHP GFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLKER SGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYIRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCYFF TIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQEAYFVSESFEEAKEKMRDFAKSITRPFSVYFNPY |
| Q8IWU9-2 | MQPAMMMFSSKYWARRGFSLDSAVPEEHQLLGSSTLNKPNSGKNDDKGNKGSSKREAATESGKTAVVFSLKNEVGGLVKALRLFQEKRVN MVHIESRKSRRRSSEVEIFVDCECGKTEFNELIQLLKFQTTIVTLNPPENIWTEEEGKELEDVPWFPRKISELDKCSHRVLMYGSELDAD HPGFKDNVYRQRRKYFVDVAMGYKYGQPIPRVEYTEEETKTWGVVFRELSKLYPTHACREYLKNFPLLTKYCGYREDNVPQLEDVSMFLK ERSGFTVRPVAGYLSPRDFLAGLAYRVFHCTQYIRHGSDPLYTPEPDTCHELLGHVPLLADPKFAQFSQEIGLASLGASDEDVQKLATCY FFTIEFGLCKQEGQLRAYGAGLLSSIGELKHALSDKACVKAFDPKTTCLQECLITTFQEAYFVSESFEEAKEKMRDFAKSITRPFSVYFN |
Protein Functional Features |
| Main function of this protein. (from UniProt) |
| TPH2 (go to UniProt):Q8IWU9 |
| Retention analysis result of protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
Gene Isoform Structures and Expression Levels for TPH2 |
| Gene structures of our canonical and alternative spliced genes of TPH2 * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Expression levels of gene isoforms across GTEx. |
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| Expression levels of gene isoforms across TCGA. |
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Protein Structures |
| PDB and CIF files of the predicted protein structures * Here we show the 3D structure of the proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
| 3D view using mol* of Q8IWU9-1 |
| 3D view using mol* of Q8IWU9-2 |
pLDDT Score Distribution |
| pLDDT score distribution of the predicted protein structures from AlphaFold2 * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
| pLDDT distribution across the protein length of Q8IWU9-1 |
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| pLDDT distribution across the protein length of Q8IWU9-2 |
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Ramachandran Plot of Protein Structures |
| Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this protein peptide. |
| Ramachandran plot of Q8IWU9-1 |
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| Ramachandran plot of Q8IWU9-2 |
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Potential Active Site Information |
| The potential binding sites of these proteins were identified using SiteMap, a module of the Schrodinger suite. |
| UniProt-id | Site score | Size | D score | Volume | Exposure | Enclosure | Contact | Phobic | Philic | Balance | Don/Acc | Residues |
| Q8IWU9-1 | 1.014 | 177 | 1.056 | 577.612 | 0.634 | 0.674 | 0.801 | 0.519 | 0.886 | 0.586 | 1.018 | 63,138,139,140,141,142,143,144,145,146,147,148,149 ,150,151,152,153,154,156,157,158,160,163,450,451,4 52,453,454,455,456,458,462,465 |
| Q8IWU9-2 | 1.019 | 171 | 1.054 | 446.243 | 0.565 | 0.694 | 0.927 | 0.698 | 0.928 | 0.752 | 1.154 | 8,9,10,12,13,15,16,17,18,19,20,21,153,154,155,156, 169,171,172,283,285,286,287,288,349,350,351,353,35 4 |
Protein Structure and Feature Comparision |
| Protein Structure Comparision Using Template Modeling Scores (TM-score). |
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| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Canonical validated structure (PDB)(green) |
| 3D view using mol* of Q8IWU9-1_Q8IWU9-1_4v06_A.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical validated structure (PDB)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of Q8IWU9-1_4v06_A_Q8IWU9-2.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of Q8IWU9-1_Q8IWU9-2.pdb |
| Protein Feature Comparison of the protein sequendary structures among the protiens. |
| ./stats/secondary_structure/figure/Q8IWU9-1_vs_Q8IWU9-2.png |
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| Protein Feature Comparison of the relative accessible surface area (ASA) among the protiens. |
| ./stats/relative_asa/Q8IWU9-1_vs_Q8IWU9-2.png |
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Protein-Protein Interaction |
| Interactors from UniProt. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| Interactors from STRING. |
| Gene name | Interactors |
Related Drugs to TPH2 |
| Drugs targeting this gene/protein. (DrugBank) |
| UniProt accession | Gene name | DrugBank ID | Drug name | Drug group | Actions |
| Q8IWU9 | TPH2 | DB12095 | Telotristat ethyl | approved, investigational | antagonist |
Related Diseases to TPH2 |
| Previous studies relating to the alternative splicing of TPH2 and disease information from the MeSH term (PubMed) |
| Gene | PMID | Title | Abstract | MeSH ID | MeSH term |
| TPH2 | 20856248 | A functional alternative splicing mutation in human tryptophan hydroxylase-2. | The brain serotonergic system has an essential role in the physiological functions of the central nervous system and dysregulation of serotonin (5-HT) homeostasis has been implicated in many neuropsychiatric disorders. The tryptophan hydroxylase-2 (TPH2) gene is the rate-limiting enzyme in brain 5-HT synthesis, and thus is an ideal candidate gene for understanding the role of dysregulation of brain serotonergic homeostasis. Here, we characterized a common, but functional single-nucleotide polymorphism (SNP rs1386493) in the TPH2 gene, which decreases efficiency of normal RNA splicing, resulting in a truncated TPH2 protein (TPH2-TR) by alternative splicing. TPH2-TR, which lacks TPH2 enzyme activity, dominant-negatively affects full-length TPH2 function, causing reduced 5-HT production. The predicted mRNA for TPH2-TR is present in postmortem brain of rs1386493 carriers. The rs13864923 variant does not appear to be overrepresented in either global or multiplex depression cohorts. However, in combination with other gene variants linked to 5-HT homeostasis, this variant may exhibit important epistatic influences. | D020022 | Genetic Predisposition to Disease |
Clinically important variants in TPH2 |
| (ClinVar, 04/20/2024) |
| accession_id | uniprot_id | gene_name | Type | Variant | Clinical_significance |
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