ASpdb: an integrative knowledgebase of human protein isoforms from experimental and AI-predicted structures

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	Protein Summary
	AS Summary
	Protein Functional Features
	Gene Isoform Structures and Expression Levels
	Protein Structures
	pLDDT Score Distribution
	Ramachandran Plot of Protein Structures
	Potential Active Site Information
	Protein Structure and Feature Comparision
	Protein-Protein Interaction
	Related Drugs
	Related Diseases
	Clinically Important Variants

Protein:CRHR2

Protein Summary

Gene summary

Gene name: CRHR2
ASpdb.0 ID: 1395
	Gene
Gene symbol	CRHR2
Gene ID	1395
Gene name	corticotropin releasing hormone receptor 2
Synonyms	CRF-RB\|CRF2\|CRFR2\|HM-CRF
Cytomap	7p14.3
Type of gene	protein-coding
Description	corticotropin-releasing factor receptor 2CRH-R2
Modification date	20240305
UniProtAcc	Q13324

Gene ontology of this gene with evidence of Inferred from Direct Assay (IDA) from Entrez

Partner	Gene	GO ID	GO term	PubMed ID
Gene	CRHR2	GO:0005886	plasma membrane	-

AS Summary

Information of the canonical protein with experimentally identified structure from PDB (2023).

UniProt Acc	File name	PDB ID	Method	Resolution	Chain	Start	End
Q13324-1	Q13324-1_3n93_A.pdb	3N93	X-ray	2.5	A	27	104

ASpdb's canonical and alternatively spliced isoform information.

accession_id

gene_name

canonical_id

alternative_id

canonical_length

alternative_length

canonical_start

canonical_end

type

originalSEQ

variationSEQ

alternative_start

alternative_end

Q13324

CRHR2

Q13324-1

Q13324-2

411

438

Substitution

MDAALLHSLLEANCSLALAEELLLDGWGPPLDPE

MRGPSGPPGLLYVPHLLLCLLCLLPPPLQYAAGQSQMPKDQPLWALLEQYCHTIMTLTNLS

Q13324

CRHR2

Q13324-1

Q13324-3

411

397

Substitution

MDAALLHSLLEANCSLALAEELLLDGWGPPLDPE

MGREPWPEDRDLGFPQLFCQ

Q13324

CRHR2

Q13324-1

Q13324-4

411

387

366

411

Substitution

VRSAVRKRWHRWQDHHSLRVPMARAMSIPTSPTRISFHSIKQTAAV

SWVSKEAQAAGPHGREKPEQRW

366

387

Q13324

CRHR2

Q13324-1

Q13324-5

411

370

366

411

Substitution

VRSAVRKRWHRWQDHHSLRVPMARAMSIPTSPTRISFHSIKQTAAV

GLEPV

366

370

Q13324

CRHR2

Q13324-1

Q13324-6

411

366

367

411

Deletion

none

366

Q13324

CRHR2

Q13324-1

Q13324-7

411

410

106

Deletion

none

105

Multiple sequence alignment of our canonical and alternatively spliced CRHR2

Matched gene isoform IDs with Ensembl and RefSeq of our canonical and alternative spliced genes of CRHR2

UniProt-id	ENSG	ENST	ENSP
Q13324-1	ENSG00000106113.19	ENST00000471646.6	ENSP00000418722.1
Q13324-2	ENSG00000106113.19	ENST00000348438.8	ENSP00000340943.4
Q13324-3	ENSG00000106113.19	ENST00000341843.8	ENSP00000344304.4
Q13324-4	ENSG00000106113.19	ENST00000506074.6	ENSP00000426498.3

UniProt-id	NM ID	NP ID
Q13324-1	NM_001883.4	NP_001874.2
Q13324-2	NM_001202475.1	NP_001189404.1
Q13324-3	NM_001202481.1	NP_001189410.1
Q13324-4	NM_001202483.1	NP_001189412.1
Q13324-7	NM_001202482.1	NP_001189411.1

Amino acid sequences of our canonical and alternatively spliced CRHR2

accession_id	Protein sequence
Q13324-1	MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNGVKYNTTRNAYRECLENGTWA SKINYSQCEPILDDKQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESN EVWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQG PIILVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYC
Q13324-2	MRGPSGPPGLLYVPHLLLCLLCLLPPPLQYAAGQSQMPKDQPLWALLEQYCHTIMTLTNLSGPYSYCNTTLDQIGTCWPRSAAGALVERP CPEYFNGVKYNTTRNAYRECLENGTWASKINYSQCEPILDDKQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLALRSIRCLRNVIHW NLITTFILRNVMWFLLQLVDHEVHESNEVWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWA IGKLYYENEQCWFGKEPGDLVDYIYQGPIILVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGED
Q13324-3	MGREPWPEDRDLGFPQLFCQGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNGVKYNTTRNAYRECLENGTWASKINYSQCEPILDD KQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESNEVWCRCITTIFNYF VVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQGPIILVLLINFVFLF NIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYCFFNGEVRSAVRKRW
Q13324-4	MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNGVKYNTTRNAYRECLENGTWA SKINYSQCEPILDDKQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESN EVWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQG PIILVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYC
Q13324-5	MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNGVKYNTTRNAYRECLENGTWA SKINYSQCEPILDDKQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESN EVWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQG PIILVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYC
Q13324-6	MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNGVKYNTTRNAYRECLENGTWA SKINYSQCEPILDDKQRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESN EVWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQG PIILVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYC
Q13324-7	MDAALLHSLLEANCSLALAEELLLDGWGPPLDPEGPYSYCNTTLDQIGTCWPRSAAGALVERPCPEYFNGVKYNTTRNAYRECLENGTWA SKINYSQCEPILDDKRKYDLHYRIALVVNYLGHCVSVAALVAAFLLFLALRSIRCLRNVIHWNLITTFILRNVMWFLLQLVDHEVHESNE VWCRCITTIFNYFVVTNFFWMFVEGCYLHTAIVMTYSTERLRKCLFLFIGWCIPFPIIVAWAIGKLYYENEQCWFGKEPGDLVDYIYQGP IILVLLINFVFLFNIVRILMTKLRASTTSETIQYRKAVKATLVLLPLLGITYMLFFVNPGEDDLSQIMFIYFNSFLQSFQGFFVSVFYCF

Protein Functional Features

Main function of this protein. (from UniProt)

CRHR2 (go to UniProt):Q13324

Retention analysis result of protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at
download page
* Minus value of BPloci means that the break pointn is located before the CDS.

- Retained protein feature among the 13 regional features.

Accession_id	Subsection	Start	End	Funcitonal feature	Splicing information
Q13324	Topological domain	1	108	Note=Extracellular;Ontology_term=ECO:0000250;evidence=ECO:0000250	Type=Substitution;Start=1;End=34
Q13324	Topological domain	1	108	Note=Extracellular;Ontology_term=ECO:0000250;evidence=ECO:0000250	Type=Substitution;Start=1;End=34
Q13324	Topological domain	1	108	Note=Extracellular;Ontology_term=ECO:0000250;evidence=ECO:0000250	Type=Deletion;Start=106;End=106
Q13324	Topological domain	365	411	Note=Cytoplasmic;Ontology_term=ECO:0000250;evidence=ECO:0000250	Type=Substitution;Start=366;End=411
Q13324	Topological domain	365	411	Note=Cytoplasmic;Ontology_term=ECO:0000250;evidence=ECO:0000250	Type=Substitution;Start=366;End=411
Q13324	Topological domain	365	411	Note=Cytoplasmic;Ontology_term=ECO:0000250;evidence=ECO:0000250	Type=Deletion;Start=367;End=411

Gene Isoform Structures and Expression Levels for CRHR2

Gene structures of our canonical and alternative spliced genes of CRHR2
* Click on the image to open the UCSC genome browser with custom track showing this image in a new window.

Expression levels of gene isoforms across GTEx.

Expression levels of gene isoforms across TCGA.

Protein Structures

PDB and CIF files of the predicted protein structures
* Here we show the 3D structure of the proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format.

3D view using mol* of Q13324-1

3D view using mol* of Q13324-2

3D view using mol* of Q13324-3

3D view using mol* of Q13324-4

3D view using mol* of Q13324-5

3D view using mol* of Q13324-6

3D view using mol* of Q13324-7

pLDDT Score Distribution

pLDDT score distribution of the predicted protein structures from AlphaFold2
* AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100.

pLDDT distribution across the protein length of Q13324-1

pLDDT distribution across the protein length of Q13324-2

pLDDT distribution across the protein length of Q13324-3

pLDDT distribution across the protein length of Q13324-4

pLDDT distribution across the protein length of Q13324-5

pLDDT distribution across the protein length of Q13324-6

pLDDT distribution across the protein length of Q13324-7

Ramachandran Plot of Protein Structures

Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this protein peptide.

Ramachandran plot of Q13324-1

Ramachandran plot of Q13324-2

Ramachandran plot of Q13324-3

Ramachandran plot of Q13324-4

Potential Active Site Information

The potential binding sites of these proteins were identified using SiteMap, a module of the Schrodinger suite.

UniProt-id	Site score	Size	D score	Volume	Exposure	Enclosure	Contact	Phobic	Philic	Balance	Don/Acc	Residues
Q13324-1	1.073	125	1.109	431.494	0.586	0.762	0.976	1.337	0.884	1.513	0.735	120,124,162,163,166,169,188,191,192,195,198,199,24 2,246,255,256,257,258,259,260,261,262,265,268,269, 272,273,276,323,326,327,348
Q13324-2	1.114	77	1.124	125.881	0.403	0.935	1.347	2.615	0.803	3.258	0.829	179,226,229,230,232,233,236,302,303,304,306,307,31 0,313,314,340,343,344,346,347,348,351,382,386
Q13324-3	1.117	71	1.127	111.132	0.388	0.958	1.332	2.8	0.736	3.803	0.773	141,145,188,191,192,195,262,265,266,269,272,273,29 9,302,303,305,306,307,310,341,345
Q13324-4	1.133	122	1.179	428.407	0.52	0.822	1.128	1.806	0.778	2.322	1.286	137,138,141,148,149,152,205,208,209,213,286,290,30 5,308,309,312,313,315,316,319,354,355,358,359,360, 362,363,364,365,366,367
Q13324-5	1.186	89	1.277	139.944	0.433	0.838	1.131	3.222	0.346	9.325	1.82	155,202,205,206,209,276,277,279,280,282,283,286,31 6,317,319,320,321,324
Q13324-6	1.277	98	1.361	142.002	0.3	0.943	1.311	3.816	0.417	9.153	1.375	152,202,205,206,209,276,277,279,280,282,283,286,31 6,317,319,320,321,324,359
Q13324-7	1.235	110	1.287	149.891	0.312	0.944	1.348	2.978	0.674	4.421	0.901	154,198,201,204,205,207,208,275,276,278,279,281,28 2,285,286,312,314,315,316,318,319,320,323,353,354, 357,358

Protein Structure and Feature Comparision

Protein Structure Comparision Using Template Modeling Scores (TM-score).

Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Canonical validated structure (PDB)(green)

3D view using mol* of Q13324-1_Q13324-1_3n93_A.pdb

Protein Structure Comparision Visualization with mol*. between Canonical validated structure (PDB)(orange) vs Alternative predicted structure (AF2)(green)

3D view using mol* of Q13324-1_3n93_A_Q13324-2.pdb

3D view using mol* of Q13324-1_3n93_A_Q13324-3.pdb

3D view using mol* of Q13324-1_3n93_A_Q13324-4.pdb

3D view using mol* of Q13324-1_3n93_A_Q13324-5.pdb

3D view using mol* of Q13324-1_3n93_A_Q13324-6.pdb

3D view using mol* of Q13324-1_3n93_A_Q13324-7.pdb

Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Alternative predicted structure (AF2)(green)

3D view using mol* of Q13324-1_Q13324-2.pdb

3D view using mol* of Q13324-1_Q13324-3.pdb

3D view using mol* of Q13324-1_Q13324-4.pdb

3D view using mol* of Q13324-1_Q13324-5.pdb

3D view using mol* of Q13324-1_Q13324-6.pdb

3D view using mol* of Q13324-1_Q13324-7.pdb

Protein Feature Comparison of the protein sequendary structures among the protiens.

./stats/secondary_structure/figure/Q13324-1_vs_Q13324-2.png

./stats/secondary_structure/figure/Q13324-1_vs_Q13324-3.png

./stats/secondary_structure/figure/Q13324-1_vs_Q13324-4.png

./stats/secondary_structure/figure/Q13324-1_vs_Q13324-5.png

./stats/secondary_structure/figure/Q13324-1_vs_Q13324-6.png

./stats/secondary_structure/figure/Q13324-1_vs_Q13324-7.png

Protein Feature Comparison of the relative accessible surface area (ASA) among the protiens.

./stats/relative_asa/Q13324-1_vs_Q13324-2.png

./stats/relative_asa/Q13324-1_vs_Q13324-3.png

./stats/relative_asa/Q13324-1_vs_Q13324-4.png

./stats/relative_asa/Q13324-1_vs_Q13324-5.png

./stats/relative_asa/Q13324-1_vs_Q13324-6.png

./stats/relative_asa/Q13324-1_vs_Q13324-7.png

Protein-Protein Interaction

Interactors from UniProt.

Accession_id

Subsection

Start

End

Funcitonal feature

Splicing information

Interactors from STRING.

Gene name

Interactors

Related Drugs to CRHR2

Drugs targeting this gene/protein.
(DrugBank)

UniProt accession

Gene name

DrugBank ID

Drug name

Drug group

Actions

Related Diseases to CRHR2

Previous studies relating to the alternative splicing of CRHR2 and disease information from the MeSH term (PubMed)

Gene

PMID

Title

Abstract

MeSH ID

MeSH term

Clinically important variants in CRHR2

(ClinVar, 04/20/2024)

accession_id	uniprot_id	gene_name	Type	Variant	Clinical_significance
Q13324	Q13324-2	CRHR2	single nucleotide variant	p.Gln36His	Uncertain significance
Q13324	Q13324-2	CRHR2	single nucleotide variant	p.Gln36His	Uncertain significance