Protein:ERN1 |
Protein Summary |
 Gene summary |
| Gene name: ERN1 | ASpdb.0 ID: 2081 | Gene | Gene symbol | ERN1 | Gene ID | 2081 |
| Gene name | endoplasmic reticulum to nucleus signaling 1 |
| Synonyms | IRE1|IRE1P|IRE1a|hIRE1p |
| Cytomap | 17q23.3 |
| Type of gene | protein-coding |
| Description | serine/threonine-protein kinase/endoribonuclease IRE1ER to nucleus signalling 1inositol-requiring 1inositol-requiring enzyme 1inositol-requiring protein 1ire1-alphaprotein kinase/endoribonuclease |
| Modification date | 20240416 |
| UniProtAcc | O75460 |
| Gene ontology of this gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Gene | ERN1 | GO:0000287 | magnesium ion binding | 9637683 |
| Gene | ERN1 | GO:0001935 | endothelial cell proliferation | 23529610 |
| Gene | ERN1 | GO:0004521 | RNA endonuclease activity | 9637683|11779465|19328063|21317875 |
| Gene | ERN1 | GO:0004674 | protein serine/threonine kinase activity | 9637683|21317875 |
| Gene | ERN1 | GO:0005524 | ATP binding | 9637683 |
| Gene | ERN1 | GO:0005737 | cytoplasm | 19622636 |
| Gene | ERN1 | GO:0005783 | endoplasmic reticulum | 23529610 |
| Gene | ERN1 | GO:0005789 | endoplasmic reticulum membrane | 9637683 |
| Gene | ERN1 | GO:0006468 | protein phosphorylation | 9637683 |
| Gene | ERN1 | GO:0033120 | positive regulation of RNA splicing | 11779464|19622636 |
| Gene | ERN1 | GO:0034620 | cellular response to unfolded protein | 19328063 |
| Gene | ERN1 | GO:0034976 | response to endoplasmic reticulum stress | 10650002 |
| Gene | ERN1 | GO:0035924 | cellular response to vascular endothelial growth factor stimulus | 23529610 |
| Gene | ERN1 | GO:0036289 | peptidyl-serine autophosphorylation | 20103773 |
| Gene | ERN1 | GO:0036498 | IRE1-mediated unfolded protein response | 9637683|11779465|19328063|29198525 |
| Gene | ERN1 | GO:0042803 | protein homodimerization activity | 29198525 |
| Gene | ERN1 | GO:0043507 | positive regulation of JUN kinase activity | 10650002 |
| Gene | ERN1 | GO:0043531 | ADP binding | 21317875 |
| Gene | ERN1 | GO:0046777 | protein autophosphorylation | 9637683|19328063 |
| Gene | ERN1 | GO:0051879 | Hsp90 protein binding | 12446770 |
| Gene | ERN1 | GO:0070054 | mRNA splicing, via endonucleolytic cleavage and ligation | 11779464|19328063|19622636|21317875 |
| Gene | ERN1 | GO:0071333 | cellular response to glucose stimulus | 20103773 |
| Gene | ERN1 | GO:1901142 | insulin metabolic process | 20103773 |
| Gene | ERN1 | GO:1990604 | IRE1-TRAF2-ASK1 complex | 23000344 |
| Gene | ERN1 | GO:1990630 | IRE1-RACK1-PP2A complex | 20103773 |
AS Summary |
| Information of the canonical protein with experimentally identified structure from PDB (2023). |
| UniProt Acc | File name | PDB ID | Method | Resolution | Chain | Start | End |
| O75460-1 | O75460-1_6w3c_A.pdb | 6W3C | X-ray | 2.3 | A | 560 | 964 |
| ASpdb's canonical and alternatively spliced isoform information. |
| accession_id | gene_name | canonical_id | alternative_id | canonical_length | alternative_length | canonical_start | canonical_end | type | originalSEQ | variationSEQ | alternative_start | alternative_end |
| O75460 | ERN1 | O75460-1 | O75460-2 | 977 | 70 | 19 | 70 | Substitution | IFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEE | VSDRGAWGGGQLATAGSGPGQRRGAGAGVRAGSATAAARCPVSPAVGGSGRA | 19 | 70 |
| O75460 | ERN1 | O75460-1 | O75460-2 | 977 | 70 | 71 | 977 | Deletion | none | none | 70 | 70 |
| Multiple sequence alignment of our canonical and alternatively spliced ERN1 |
| Matched gene isoform IDs with Ensembl and RefSeq of our canonical and alternative spliced genes of ERN1 |
| UniProt-id | ENSG | ENST | ENSP |
| O75460-1 | ENSG00000178607.17 | ENST00000433197.4 | ENSP00000401445.2 |
| O75460-2 | ENSG00000178607.17 | ENST00000606895.2 | ENSP00000475519.1 |
| UniProt-id | NM ID | NP ID |
| O75460-1 | NM_001433.3 | NP_001424.3 |
| Amino acid sequences of our canonical and alternatively spliced ERN1 |
| accession_id | Protein sequence |
| O75460-1 | MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNN EGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYF DYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPF PKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWL LIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILS TFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSG SSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFC TEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAV GRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIE KMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDL |
| O75460-2 |
Protein Functional Features |
| Main function of this protein. (from UniProt) |
| ERN1 (go to UniProt):O75460 |
| Retention analysis result of protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| O75460 | Topological domain | 19 | 443 | Note=Lumenal;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Substitution;Start=19;End=70 |
| O75460 | Topological domain | 19 | 443 | Note=Lumenal;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=71;End=977 |
| O75460 | Transmembrane | 444 | 464 | Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=71;End=977 |
| O75460 | Topological domain | 465 | 977 | Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=71;End=977 |
| O75460 | Domain | 571 | 832 | Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159 | Type=Deletion;Start=71;End=977 |
| O75460 | Domain | 835 | 963 | Note=KEN;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00725 | Type=Deletion;Start=71;End=977 |
| O75460 | Region | 410 | 434 | Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite | Type=Deletion;Start=71;End=977 |
| O75460 | Region | 491 | 559 | Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite | Type=Deletion;Start=71;End=977 |
| O75460 | Region | 906 | 907 | Note=Interacts with hydroxy-aryl-aldehyde inhibitors;Ontology_term=ECO:0000250;evidence=ECO:0000250|UniProtKB:Q9EQY0 | Type=Deletion;Start=71;End=977 |
| O75460 | Compositional bias | 511 | 551 | Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite | Type=Deletion;Start=71;End=977 |
Gene Isoform Structures and Expression Levels for ERN1 |
| Gene structures of our canonical and alternative spliced genes of ERN1 * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Expression levels of gene isoforms across GTEx. |
 |
| Expression levels of gene isoforms across TCGA. |
 |
Protein Structures |
| PDB and CIF files of the predicted protein structures * Here we show the 3D structure of the proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
| 3D view using mol* of O75460-1 |
| 3D view using mol* of O75460-2 |
pLDDT Score Distribution |
| pLDDT score distribution of the predicted protein structures from AlphaFold2 * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
| pLDDT distribution across the protein length of O75460-1 |
 |
Ramachandran Plot of Protein Structures |
| Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this protein peptide. |
| Ramachandran plot of O75460-1 |
 |
| Ramachandran plot of O75460-2 |
 |
Potential Active Site Information |
| The potential binding sites of these proteins were identified using SiteMap, a module of the Schrodinger suite. |
| UniProt-id | Site score | Size | D score | Volume | Exposure | Enclosure | Contact | Phobic | Philic | Balance | Don/Acc | Residues |
| O75460-1 | 1.064 | 426 | 1.11 | 1550.017 | 0.558 | 0.731 | 0.9 | 0.738 | 0.822 | 0.898 | 1.043 | 76,77,78,79,81,83,95,99,101,102,105,159,161,179,18 1,182,183,184,186,187,188,191,193,195,196,197,198, 227,228,230,231,233,238,239,240,241,242,243,244,25 3,266,268,269,270,271,272,276,278,279,280,282,297, 298,299,309,367,383,384,385,386,387,388,389,390,39 1,392,393,394,395,396,397,399,400,401,403,404 |
Protein Structure and Feature Comparision |
| Protein Structure Comparision Using Template Modeling Scores (TM-score). |
 |
| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Canonical validated structure (PDB)(green) |
| 3D view using mol* of O75460-1_O75460-1_6w3c_A.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical validated structure (PDB)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of O75460-1_6w3c_A_O75460-2.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of O75460-1_O75460-2.pdb |
| Protein Feature Comparison of the protein sequendary structures among the protiens. |
| ./stats/secondary_structure/figure/O75460-1_vs_O75460-2.png |
 < |
| Protein Feature Comparison of the relative accessible surface area (ASA) among the protiens. |
| ./stats/relative_asa/O75460-1_vs_O75460-2.png |
 < |
Protein-Protein Interaction |
| Interactors from UniProt. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| Interactors from STRING. |
| Gene name | Interactors |
Related Drugs to ERN1 |
| Drugs targeting this gene/protein. (DrugBank) |
| UniProt accession | Gene name | DrugBank ID | Drug name | Drug group | Actions |
| O75460 | ERN1 | DB12010 | Fostamatinib | approved, investigational | inhibitor |
| O75460 | ERN1 | DB07382 | N~2~-1H-benzimidazol-5-yl-N~4~-(3-cyclopropyl-1H-pyrazol-5-yl)pyrimidine-2,4-diamine | experimental |
Related Diseases to ERN1 |
| Previous studies relating to the alternative splicing of ERN1 and disease information from the MeSH term (PubMed) |
| Gene | PMID | Title | Abstract | MeSH ID | MeSH term |
Clinically important variants in ERN1 |
| (ClinVar, 04/20/2024) |
| accession_id | uniprot_id | gene_name | Type | Variant | Clinical_significance |
Copyright 2024-PresentThe University of Texas Health Science Center at Houston (UTHealth Houston) Web File Viewing | Emergency Information |