Protein:APAF1 |
Protein Summary |
 Gene summary |
| Gene name: APAF1 | ASpdb.0 ID: 317 | Gene | Gene symbol | APAF1 | Gene ID | 317 |
| Gene name | apoptotic peptidase activating factor 1 |
| Synonyms | APAF-1|CED4 |
| Cytomap | 12q23.1 |
| Type of gene | protein-coding |
| Description | apoptotic protease-activating factor 1 |
| Modification date | 20240407 |
| UniProtAcc | O14727 |
| Gene ontology of this gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Gene | APAF1 | GO:0006919 | activation of cysteine-type endopeptidase activity involved in apoptotic process | 21827945 |
| Gene | APAF1 | GO:0008635 | activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c | 9267021 |
| Gene | APAF1 | GO:0032991 | protein-containing complex | 11821383 |
| Gene | APAF1 | GO:0043293 | apoptosome | 21827945 |
AS Summary |
| Information of the canonical protein with experimentally identified structure from PDB (2023). |
| UniProt Acc | File name | PDB ID | Method | Resolution | Chain | Start | End |
| O14727-1 | O14727-1_5juy_B.pdb | 5JUY | EM | 4.1 | B | 1 | 1248 |
| ASpdb's canonical and alternatively spliced isoform information. |
| accession_id | gene_name | canonical_id | alternative_id | canonical_length | alternative_length | canonical_start | canonical_end | type | originalSEQ | variationSEQ | alternative_start | alternative_end |
| O14727 | APAF1 | O14727-1 | O14727-4 | 1248 | 1205 | 824 | 866 | Deletion | none | none | 823 | 823 |
| O14727 | APAF1 | O14727-1 | O14727-6 | 1248 | 338 | 319 | 338 | Substitution | GSPLVVSLIGALLRDFPNRW | VVERCHWGILTDLLHKWNQS | 319 | 338 |
| O14727 | APAF1 | O14727-1 | O14727-6 | 1248 | 338 | 339 | 1248 | Deletion | none | none | 338 | 338 |
| Multiple sequence alignment of our canonical and alternatively spliced APAF1 |
| Matched gene isoform IDs with Ensembl and RefSeq of our canonical and alternative spliced genes of APAF1 |
| UniProt-id | ENSG | ENST | ENSP |
| O14727-1 | ENSG00000120868.14 | ENST00000551964.6 | ENSP00000448165.2 |
| O14727-4 | ENSG00000120868.14 | ENST00000357310.5 | ENSP00000349862.1 |
| O14727-4 | ENSG00000120868.14 | ENST00000547045.5 | ENSP00000449791.1 |
| O14727-6 | ENSG00000120868.14 | ENST00000333991.5 | ENSP00000334558.1 |
| O14727-6 | ENSG00000120868.14 | ENST00000552268.5 | ENSP00000448826.1 |
| UniProt-id | NM ID | NP ID |
| O14727-1 | NM_181861.1 | NP_863651.1 |
| O14727-4 | NM_181868.1 | NP_863658.1 |
| O14727-6 | NM_181869.1 | NP_863659.1 |
| Amino acid sequences of our canonical and alternatively spliced APAF1 |
| accession_id | Protein sequence |
| O14727-1 | MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDG IPVVSSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPG GVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVT DSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYD YEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNC SQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAV SENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFSEDGQRI ASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGS SDCFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDMEVIVKCCSWSADG ARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTS SDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILEL VNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKD FVCHQGTVLSCDISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGELLHLCAPLSE |
| O14727-4 | MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDG IPVVSSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPG GVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVT DSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYD YEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNC SQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAV SENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFSEDGQRI ASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGS SDCFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDMEVIVKCCSWSADG ARIMVAAKNKIFLWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHI RRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQ LDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHDATKFSSTSADKTAKIWSFDLLLPLHEL RGHNGCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNVVTGESSQTF |
| O14727-6 | MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDG IPVVSSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPG GVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVT |
Protein Functional Features |
| Main function of this protein. (from UniProt) |
| APAF1 (go to UniProt):O14727 |
| Retention analysis result of protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| O14727 | Domain | 104 | 415 | Note=NB-ARC | Type=Substitution;Start=319;End=338 |
| O14727 | Domain | 104 | 415 | Note=NB-ARC | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 613 | 652 | Note=WD 1-1 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 655 | 694 | Note=WD 1-2 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 697 | 738 | Note=WD 1-3 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 741 | 780 | Note=WD 1-4 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 796 | 836 | Note=WD 1-5 | Type=Deletion;Start=824;End=866 |
| O14727 | Repeat | 796 | 836 | Note=WD 1-5 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 838 | 877 | Note=WD 1-6 | Type=Deletion;Start=824;End=866 |
| O14727 | Repeat | 838 | 877 | Note=WD 1-6 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 880 | 910 | Note=WD 1-7 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 922 | 958 | Note=WD 2-1 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 959 | 998 | Note=WD 2-2 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 1001 | 1040 | Note=WD 2-3 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 1042 | 1080 | Note=WD 2-4 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 1083 | 1122 | Note=WD 2-5 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 1125 | 1164 | Note=WD 2-6 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 1175 | 1212 | Note=WD 2-7 | Type=Deletion;Start=339;End=1248 |
| O14727 | Repeat | 1213 | 1248 | Note=WD 2-8 | Type=Deletion;Start=339;End=1248 |
| O14727 | Region | 910 | 921 | Note=Interpropeller linker;Ontology_term=ECO:0000250;evidence=ECO:0000250 | Type=Deletion;Start=339;End=1248 |
Gene Isoform Structures and Expression Levels for APAF1 |
| Gene structures of our canonical and alternative spliced genes of APAF1 * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Expression levels of gene isoforms across GTEx. |
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| Expression levels of gene isoforms across TCGA. |
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Protein Structures |
| PDB and CIF files of the predicted protein structures * Here we show the 3D structure of the proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
| 3D view using mol* of O14727-1 |
| 3D view using mol* of O14727-4 |
| 3D view using mol* of O14727-6 |
pLDDT Score Distribution |
| pLDDT score distribution of the predicted protein structures from AlphaFold2 * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
| pLDDT distribution across the protein length of O14727-1 |
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| pLDDT distribution across the protein length of O14727-4 |
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| pLDDT distribution across the protein length of O14727-6 |
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Ramachandran Plot of Protein Structures |
| Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this protein peptide. |
| Ramachandran plot of O14727-1 |
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| Ramachandran plot of O14727-4 |
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| Ramachandran plot of O14727-6 |
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Potential Active Site Information |
| The potential binding sites of these proteins were identified using SiteMap, a module of the Schrodinger suite. |
| UniProt-id | Site score | Size | D score | Volume | Exposure | Enclosure | Contact | Phobic | Philic | Balance | Don/Acc | Residues |
| O14727-1 | 1.134 | 302 | 1.063 | 529.592 | 0.315 | 0.899 | 1.167 | 0.877 | 1.279 | 0.686 | 0.851 | 120,121,122,123,124,125,126,127,128,129,133,156,15 7,158,159,160,161,162,163,165,166,169,184,186,187, 188,243,244,265,286,294,298,321,322,325,392,394,41 9,422,424,425,426,427,436,437,438,439 |
| O14727-4 | 1.102 | 378 | 1.055 | 669.536 | 0.323 | 0.851 | 1.109 | 0.969 | 1.212 | 0.799 | 0.783 | 106,110,111,114,115,119,120,121,122,123,124,125,12 6,127,129,156,157,158,159,160,161,162,163,165,166, 169,184,186,188,243,244,263,265,286,294,298,321,32 2,325,389,390,391,392,394,416,419,422,424,425,426, 427,428,433,436,437,438,439,442 |
| O14727-6 | 1.146 | 216 | 1.188 | 371.469 | 0.347 | 0.847 | 1.096 | 1.657 | 0.799 | 2.074 | 1.199 | 123,125,126,127,128,129,155,156,157,158,159,162,16 3,283,284,285,286,287,288,290,291,294,295,314,317, 318,320,321,322,323,324,325,328 |
Protein Structure and Feature Comparision |
| Protein Structure Comparision Using Template Modeling Scores (TM-score). |
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| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Canonical validated structure (PDB)(green) |
| 3D view using mol* of O14727-1_O14727-1_5juy_B.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical validated structure (PDB)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of O14727-1_5juy_B_O14727-4.pdb |
| 3D view using mol* of O14727-1_5juy_B_O14727-6.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of O14727-1_O14727-4.pdb |
| 3D view using mol* of O14727-1_O14727-6.pdb |
| Protein Feature Comparison of the protein sequendary structures among the protiens. |
| ./stats/secondary_structure/figure/O14727-1_vs_O14727-4.png |
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| ./stats/secondary_structure/figure/O14727-1_vs_O14727-6.png |
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| Protein Feature Comparison of the relative accessible surface area (ASA) among the protiens. |
| ./stats/relative_asa/O14727-1_vs_O14727-4.png |
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| ./stats/relative_asa/O14727-1_vs_O14727-6.png |
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Protein-Protein Interaction |
| Interactors from UniProt. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| Interactors from STRING. |
| Gene name | Interactors |
Related Drugs to APAF1 |
| Drugs targeting this gene/protein. (DrugBank) |
| UniProt accession | Gene name | DrugBank ID | Drug name | Drug group | Actions |
| O14727 | APAF1 | DB00171 | ATP | investigational, nutraceutical |
Related Diseases to APAF1 |
| Previous studies relating to the alternative splicing of APAF1 and disease information from the MeSH term (PubMed) |
| Gene | PMID | Title | Abstract | MeSH ID | MeSH term |
| APAF1 | 12804598 | APAF-1-ALT, a novel alternative splicing form of APAF-1, potentially causes impeded ability of undergoing DNA damage-induced apoptosis in the LNCaP human prostate cancer cell line. | We have found a novel alternative splicing product of the apoptotic protease activating factor 1 (APAF-1), termed APAF-1-ALT, in the LNCaP human prostate cancer cell line. APAF-1-ALT harbors the caspase recruitment domain and an incomplete CED-4 like/ATPase domain, but lacks the WD-40 repeat units. The LNCaP cell expressed the full-length APAF-1 weakly and APAF-1-ALT rather abundantly, especially after mycoplasma infection. LNCaP underwent a retarded DNA damage-induced apoptosis, which was independent of caspase 9 activity. APAF-1-ALT functioned less effectively in inducing apoptosis than did APAF-1-XL, the full-length APAF-1, in transient transfection. Moreover, APAF-1-ALT interfered with APAF-1-XL's ability to induce apoptosis in transient double transfection experiment. These results indicate that APAF-1-ALT is a molecule that is deficient and impeded for mediating apoptosis and that it may contribute to the resistance to DNA damage-induced treatment observed in LNCaP. | D011471 | Prostatic Neoplasms |
Clinically important variants in APAF1 |
| (ClinVar, 04/20/2024) |
| accession_id | uniprot_id | gene_name | Type | Variant | Clinical_significance |
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