Protein:ROR1 |
Protein Summary |
 Gene summary |
| Gene name: ROR1 | ASpdb.0 ID: 4919 | Gene | Gene symbol | ROR1 | Gene ID | 4919 |
| Gene name | receptor tyrosine kinase like orphan receptor 1 |
| Synonyms | NTRKR1|dJ537F10.1 |
| Cytomap | 1p31.3 |
| Type of gene | protein-coding |
| Description | inactive tyrosine-protein kinase transmembrane receptor ROR1neurotrophic tyrosine kinase, receptor-related 1 |
| Modification date | 20240411 |
| UniProtAcc | Q01973 |
| Gene ontology of this gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Gene | ROR1 | GO:0005886 | plasma membrane | 27162350 |
| Gene | ROR1 | GO:0043235 | receptor complex | 23382219 |
AS Summary |
| Information of the canonical protein with experimentally identified structure from PDB (2023). |
| UniProt Acc | File name | PDB ID | Method | Resolution | Chain | Start | End |
| Q01973-1 | Q01973-1_6tu9_A.pdb | 6TU9 | X-ray | 1.94 | A | 460 | 749 |
| ASpdb's canonical and alternatively spliced isoform information. |
| accession_id | gene_name | canonical_id | alternative_id | canonical_length | alternative_length | canonical_start | canonical_end | type | originalSEQ | variationSEQ | alternative_start | alternative_end |
| Q01973 | ROR1 | Q01973-1 | Q01973-2 | 937 | 388 | 1 | 549 | Deletion | none | none | 0 | 0 |
| Q01973 | ROR1 | Q01973-1 | Q01973-3 | 937 | 393 | 392 | 393 | Substitution | DS | GK | 392 | 393 |
| Q01973 | ROR1 | Q01973-1 | Q01973-3 | 937 | 393 | 394 | 937 | Deletion | none | none | 393 | 393 |
| Multiple sequence alignment of our canonical and alternatively spliced ROR1 |
| Matched gene isoform IDs with Ensembl and RefSeq of our canonical and alternative spliced genes of ROR1 |
| UniProt-id | ENSG | ENST | ENSP |
| Q01973-1 | ENSG00000185483.13 | ENST00000371079.6 | ENSP00000360120.1 |
| Q01973-3 | ENSG00000185483.13 | ENST00000371080.5 | ENSP00000360121.1 |
| UniProt-id | NM ID | NP ID |
| Q01973-1 | NM_005012.3 | NP_005003.2 |
| Q01973-3 | NM_001083592.1 | NP_001077061.1 |
| Amino acid sequences of our canonical and alternatively spliced ROR1 |
| accession_id | Protein sequence |
| Q01973-1 | MHRPRRRGTRPPLLALLAALLLAARGAAAQETELSVSAELVPTSSWNISSELNKDSYLTLDEPMNNITTSLGQTAELHCKVSGNPPPTIR WFKNDAPVVQEPRRLSFRSTIYGSRLRIRNLDTTDTGYFQCVATNGKEVVSSTGVLFVKFGPPPTASPGYSDEYEEDGFCQPYRGIACAR FIGNRTVYMESLHMQGEIENQITAAFTMIGTSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEILENVLCQTEYIFARSN PMILMRLKLPNCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHTFTALRFPELNGGHS YCRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEKNKMEILYILVPSVAIPLAIALLFFFICVCRNNQKSSSAPVQRQPKHVRGQN VEMSMLNAYKPKSKAKELPLSAVRFMEELGECAFGKIYKGHLYLPGMDHAQLVAIKTLKDYNNPQQWTEFQQEASLMAELHHPNIVCLLG AVTQEQPVCMLFEYINQGDLHEFLIMRSPHSDVGCSSDEDGTVKSSLDHGDFLHIAIQIAAGMEYLSSHFFVHKDLAARNILIGEQLHVK ISDLGLSREIYSADYYRVQSKSLLPIRWMPPEAIMYGKFSSDSDIWSFGVVLWEIFSFGLQPYYGFSNQEVIEMVRKRQLLPCSEDCPPR MYSLMTECWNEIPSRRPRFKDIHVRLRSWEGLSSHTSSTTPSGGNATTQTTSLSASPVSNLSNPRYPNYMFPSQGITPQGQIAGFIGPPI PQNQRFIPINGYPIPPGYAAFPAAHYQPTGPPRVIQHCPPPKSRSPSSASGSTSTGHVTSLPSSGSNQEANIPLLPHMSIPNHPGGMGIT |
| Q01973-2 | MLFEYINQGDLHEFLIMRSPHSDVGCSSDEDGTVKSSLDHGDFLHIAIQIAAGMEYLSSHFFVHKDLAARNILIGEQLHVKISDLGLSRE IYSADYYRVQSKSLLPIRWMPPEAIMYGKFSSDSDIWSFGVVLWEIFSFGLQPYYGFSNQEVIEMVRKRQLLPCSEDCPPRMYSLMTECW NEIPSRRPRFKDIHVRLRSWEGLSSHTSSTTPSGGNATTQTTSLSASPVSNLSNPRYPNYMFPSQGITPQGQIAGFIGPPIPQNQRFIPI NGYPIPPGYAAFPAAHYQPTGPPRVIQHCPPPKSRSPSSASGSTSTGHVTSLPSSGSNQEANIPLLPHMSIPNHPGGMGITVFGNKSQKP |
| Q01973-3 | MHRPRRRGTRPPLLALLAALLLAARGAAAQETELSVSAELVPTSSWNISSELNKDSYLTLDEPMNNITTSLGQTAELHCKVSGNPPPTIR WFKNDAPVVQEPRRLSFRSTIYGSRLRIRNLDTTDTGYFQCVATNGKEVVSSTGVLFVKFGPPPTASPGYSDEYEEDGFCQPYRGIACAR FIGNRTVYMESLHMQGEIENQITAAFTMIGTSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEILENVLCQTEYIFARSN PMILMRLKLPNCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHTFTALRFPELNGGHS |
Protein Functional Features |
| Main function of this protein. (from UniProt) |
| ROR1 (go to UniProt):Q01973 |
| Retention analysis result of protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| Q01973 | Topological domain | 30 | 406 | Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=1;End=549 |
| Q01973 | Topological domain | 30 | 406 | Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Substitution;Start=392;End=393 |
| Q01973 | Topological domain | 30 | 406 | Note=Extracellular;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=394;End=937 |
| Q01973 | Transmembrane | 407 | 427 | Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=1;End=549 |
| Q01973 | Transmembrane | 407 | 427 | Note=Helical;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=394;End=937 |
| Q01973 | Topological domain | 428 | 937 | Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=1;End=549 |
| Q01973 | Topological domain | 428 | 937 | Note=Cytoplasmic;Ontology_term=ECO:0000255;evidence=ECO:0000255 | Type=Deletion;Start=394;End=937 |
| Q01973 | Domain | 42 | 147 | Note=Ig-like C2-type | Type=Deletion;Start=1;End=549 |
| Q01973 | Domain | 165 | 299 | Note=FZ;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00090 | Type=Deletion;Start=1;End=549 |
| Q01973 | Domain | 312 | 391 | Note=Kringle;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00121 | Type=Deletion;Start=1;End=549 |
| Q01973 | Domain | 473 | 746 | Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159 | Type=Deletion;Start=1;End=549 |
| Q01973 | Domain | 473 | 746 | Note=Protein kinase;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00159 | Type=Deletion;Start=394;End=937 |
| Q01973 | Region | 753 | 779 | Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite | Type=Deletion;Start=394;End=937 |
| Q01973 | Region | 833 | 890 | Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite | Type=Deletion;Start=394;End=937 |
| Q01973 | Compositional bias | 851 | 883 | Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite | Type=Deletion;Start=394;End=937 |
Gene Isoform Structures and Expression Levels for ROR1 |
| Gene structures of our canonical and alternative spliced genes of ROR1 * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Expression levels of gene isoforms across GTEx. |
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| Expression levels of gene isoforms across TCGA. |
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Protein Structures |
| PDB and CIF files of the predicted protein structures * Here we show the 3D structure of the proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
| 3D view using mol* of Q01973-1 |
| 3D view using mol* of Q01973-2 |
| 3D view using mol* of Q01973-3 |
pLDDT Score Distribution |
| pLDDT score distribution of the predicted protein structures from AlphaFold2 * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
| pLDDT distribution across the protein length of Q01973-1 |
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| pLDDT distribution across the protein length of Q01973-2 |
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| pLDDT distribution across the protein length of Q01973-3 |
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Ramachandran Plot of Protein Structures |
| Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this protein peptide. |
| Ramachandran plot of Q01973-1 |
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| Ramachandran plot of Q01973-3 |
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Potential Active Site Information |
| The potential binding sites of these proteins were identified using SiteMap, a module of the Schrodinger suite. |
| UniProt-id | Site score | Size | D score | Volume | Exposure | Enclosure | Contact | Phobic | Philic | Balance | Don/Acc | Residues |
| Q01973-1 | 1.049 | 420 | 1.069 | 1266.356 | 0.504 | 0.759 | 0.954 | 0.697 | 1 | 0.698 | 0.82 | 171,174,175,176,177,178,179,180,181,192,193,195,19 6,199,200,201,203,204,206,207,208,209,210,211,212, 213,214,215,216,217,220,224,266,270,272,273,276,56 1,562,565,566,568,570,571,572,573,619,639,640,641, 642,643,644,645,657,658,684,689,690,691,692,693,69 4,695,696,697,698,814,815,816,817,818,819,820,821, 822 |
| Q01973-2 | 0.897 | 58 | 0.888 | 179.046 | 0.517 | 0.754 | 1.013 | 0.923 | 0.861 | 1.072 | 1.026 | 21,40,43,134,137,138,139,141,145,163,164,165,166,1 67,273,274,276,279 |
| Q01973-3 | 1.184 | 63 | 1.267 | 77.518 | 0.33 | 0.936 | 1.333 | 6.025 | 0.125 | 48.281 | 4.653 | 206,209,210,214,215,219,223,224,227,260,262,263,26 4,266,267,273,277,279 |
Protein Structure and Feature Comparision |
| Protein Structure Comparision Using Template Modeling Scores (TM-score). |
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| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Canonical validated structure (PDB)(green) |
| 3D view using mol* of Q01973-1_Q01973-1_6tu9_A.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical validated structure (PDB)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of Q01973-1_6tu9_A_Q01973-2.pdb |
| 3D view using mol* of Q01973-1_6tu9_A_Q01973-3.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of Q01973-1_Q01973-2.pdb |
| 3D view using mol* of Q01973-1_Q01973-3.pdb |
| Protein Feature Comparison of the protein sequendary structures among the protiens. |
| ./stats/secondary_structure/figure/Q01973-1_vs_Q01973-2.png |
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| ./stats/secondary_structure/figure/Q01973-1_vs_Q01973-3.png |
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| Protein Feature Comparison of the relative accessible surface area (ASA) among the protiens. |
| ./stats/relative_asa/Q01973-1_vs_Q01973-2.png |
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| ./stats/relative_asa/Q01973-1_vs_Q01973-3.png |
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Protein-Protein Interaction |
| Interactors from UniProt. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| Interactors from STRING. |
| Gene name | Interactors |
Related Drugs to ROR1 |
| Drugs targeting this gene/protein. (DrugBank) |
| UniProt accession | Gene name | DrugBank ID | Drug name | Drug group | Actions |
Related Diseases to ROR1 |
| Previous studies relating to the alternative splicing of ROR1 and disease information from the MeSH term (PubMed) |
| Gene | PMID | Title | Abstract | MeSH ID | MeSH term |
| ROR1 | 8875995 | Human neural tissues express a truncated Ror1 receptor tyrosine kinase, lacking both extracellular and transmembrane domains. | Human heart, lung and kidney express a 6 kb mRNA encoding Ror1, a member of the receptor tyrosine kinase (RTK) family with as yet unknown ligand specificity. We used a Ror1 cDNA probe to screen a cDNA library prepared from the human neuronogenic teratocarcinoma line, NTera2, and cloned a 2373 nucleotide transcript. This transcript contains an open reading frame that encodes a 388 amino acid protein identical with the cytosolic, C-terminal region of ror1 but lacking the ror1 transmembrane and entire extracellular domains. Northern blots demonstrate that mRNA encoding this truncated Ror1 ('t-Rorl') is abundantly expressed in fetal and adult human CNS, in human leukemia, lymphoma cell lines, and in a variety of human cancers derived from neuroectoderm. While previous studies have documented alternative splicing patterns within 5' and 3' regions of mRNAs encoding various RTKs altering their ligand binding specificity or their intracellular signaling, the present report is the first to demonstrate tissue-specific alternative mRNA splicing causing loss of the entire extracellular and transmembrane regions of an RTK. | D009423 | Nervous System Neoplasms |
| ROR1 | 8875995 | Human neural tissues express a truncated Ror1 receptor tyrosine kinase, lacking both extracellular and transmembrane domains. | Human heart, lung and kidney express a 6 kb mRNA encoding Ror1, a member of the receptor tyrosine kinase (RTK) family with as yet unknown ligand specificity. We used a Ror1 cDNA probe to screen a cDNA library prepared from the human neuronogenic teratocarcinoma line, NTera2, and cloned a 2373 nucleotide transcript. This transcript contains an open reading frame that encodes a 388 amino acid protein identical with the cytosolic, C-terminal region of ror1 but lacking the ror1 transmembrane and entire extracellular domains. Northern blots demonstrate that mRNA encoding this truncated Ror1 ('t-Rorl') is abundantly expressed in fetal and adult human CNS, in human leukemia, lymphoma cell lines, and in a variety of human cancers derived from neuroectoderm. While previous studies have documented alternative splicing patterns within 5' and 3' regions of mRNAs encoding various RTKs altering their ligand binding specificity or their intracellular signaling, the present report is the first to demonstrate tissue-specific alternative mRNA splicing causing loss of the entire extracellular and transmembrane regions of an RTK. | D018243 | Teratocarcinoma |
Clinically important variants in ROR1 |
| (ClinVar, 04/20/2024) |
| accession_id | uniprot_id | gene_name | Type | Variant | Clinical_significance |
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