Protein:SLC3A2 |
Protein Summary |
 Gene summary |
| Gene name: SLC3A2 | ASpdb.0 ID: 6520 | Gene | Gene symbol | SLC3A2 | Gene ID | 6520 |
| Gene name | solute carrier family 3 member 2 |
| Synonyms | 4F2|4F2HC|4T2HC|CD98|CD98HC|MDU1|NACAE |
| Cytomap | 11q12.3 |
| Type of gene | protein-coding |
| Description | amino acid transporter heavy chain SLC3A24F2 cell-surface antigen heavy chainCD98 heavy chainantigen defined by monoclonal antibody 4F2, heavy chainantigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43lymphocyte activation antigen |
| Modification date | 20240416 |
| UniProtAcc | P08195 |
| Gene ontology of this gene with evidence of Inferred from Direct Assay (IDA) from Entrez |
| Partner | Gene | GO ID | GO term | PubMed ID |
| Gene | SLC3A2 | GO:0003725 | double-stranded RNA binding | 21266579 |
| Gene | SLC3A2 | GO:0005294 | neutral L-amino acid secondary active transmembrane transporter activity | 33298890 |
| Gene | SLC3A2 | GO:0005654 | nucleoplasm | - |
| Gene | SLC3A2 | GO:0005886 | plasma membrane | 11311135|11557028|12225859|12270127|23137377|25998567|28112518 |
| Gene | SLC3A2 | GO:0009986 | cell surface | 25063885 |
| Gene | SLC3A2 | GO:0015818 | isoleucine transport | 11311135|11557028 |
| Gene | SLC3A2 | GO:0015820 | L-leucine transport | 9751058|10574970|11557028|11564694|12225859|15769744 |
| Gene | SLC3A2 | GO:0015821 | methionine transport | 11557028 |
| Gene | SLC3A2 | GO:0015823 | phenylalanine transport | 9751058|10574970|11557028|11564694 |
| Gene | SLC3A2 | GO:0015824 | proline transport | 10574970 |
| Gene | SLC3A2 | GO:0015827 | tryptophan transport | 10574970|11557028|11564694 |
| Gene | SLC3A2 | GO:0015828 | tyrosine transport | 11557028|11564694 |
| Gene | SLC3A2 | GO:0015829 | valine transport | 11557028 |
| Gene | SLC3A2 | GO:0016323 | basolateral plasma membrane | 31791063 |
| Gene | SLC3A2 | GO:0016324 | apical plasma membrane | 31791063 |
| Gene | SLC3A2 | GO:0046982 | protein heterodimerization activity | 33298890 |
| Gene | SLC3A2 | GO:0070327 | thyroid hormone transport | 11564694 |
| Gene | SLC3A2 | GO:0140272 | exogenous protein binding | 30341327|34294905 |
| Gene | SLC3A2 | GO:1902024 | L-histidine transport | 9751058|11557028 |
| Gene | SLC3A2 | GO:1990184 | amino acid transport complex | 30867591 |
AS Summary |
| Information of the canonical protein with experimentally identified structure from PDB (2023). |
| UniProt Acc | File name | PDB ID | Method | Resolution | Chain | Start | End |
| P08195-1 | P08195-1_7ccs_A.pdb | 7CCS | EM | 6.2 | A | 162 | 630 |
| ASpdb's canonical and alternatively spliced isoform information. |
| accession_id | gene_name | canonical_id | alternative_id | canonical_length | alternative_length | canonical_start | canonical_end | type | originalSEQ | variationSEQ | alternative_start | alternative_end |
| P08195 | SLC3A2 | P08195-1 | P08195-2 | 630 | 529 | 1 | 101 | Deletion | none | none | 0 | 0 |
| P08195 | SLC3A2 | P08195-1 | P08195-3 | 630 | 568 | 38 | 99 | Deletion | none | none | 37 | 37 |
| P08195 | SLC3A2 | P08195-1 | P08195-4 | 630 | 661 | 98 | 98 | Substitution | V | VTETGFHHVSQADIEFLTSIDPTASASGSAGI | 98 | 129 |
| Multiple sequence alignment of our canonical and alternatively spliced SLC3A2 |
| Matched gene isoform IDs with Ensembl and RefSeq of our canonical and alternative spliced genes of SLC3A2 |
| UniProt-id | ENSG | ENST | ENSP |
| P08195-1 | ENSG00000168003.19 | ENST00000377890.6 | ENSP00000367122.2 |
| P08195-2 | ENSG00000168003.19 | ENST00000338663.12 | ENSP00000340815.7 |
| P08195-2 | ENSG00000168003.19 | ENST00000544377.2 | ENSP00000442135.2 |
| P08195-2 | ENSG00000168003.19 | ENST00000680631.1 | ENSP00000506006.1 |
| P08195-2 | ENSG00000168003.19 | ENST00000681657.1 | ENSP00000505110.1 |
| P08195-3 | ENSG00000168003.19 | ENST00000377889.6 | ENSP00000367121.2 |
| P08195-4 | ENSG00000168003.19 | ENST00000538084.2 | ENSP00000440001.2 |
| UniProt-id | NM ID | NP ID |
| P08195-1 | NM_002394.5 | NP_002385.3 |
| P08195-2 | NM_001013251.2 | NP_001013269.1 |
| P08195-3 | NM_001012664.2 | NP_001012682.1 |
| Amino acid sequences of our canonical and alternatively spliced SLC3A2 |
| accession_id | Protein sequence |
| P08195-1 | MELQPPEASIAVVSIPRQLPGSHSEAGVQGLSAGDDSELGSHCVAQTGLELLASGDPLPSASQNAEMIETGSDCVTQAGLQLLASSDPPA LASKNAEVTGTMSQDTEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVAEDEAEAAAAAKFTGLSKEELLKVAGSPGWVR TRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKD DVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFL AEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLL RLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGD FHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA |
| P08195-2 | MSQDTEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVAEDEAEAAAAAKFTGLSKEELLKVAGSPGWVRTRWALLLLFWL GWLGMLAGAVVIIVRAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQID PNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFS EDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLP GTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLF |
| P08195-3 | MELQPPEASIAVVSIPRQLPGSHSEAGVQGLSAGDDSGTMSQDTEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVAEDE AEAAAAAKFTGLSKEELLKVAGSPGWVRTRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLA GLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKD ALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQY LNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQ SEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPG |
| P08195-4 | MELQPPEASIAVVSIPRQLPGSHSEAGVQGLSAGDDSELGSHCVAQTGLELLASGDPLPSASQNAEMIETGSDCVTQAGLQLLASSDPPA LASKNAEVTETGFHHVSQADIEFLTSIDPTASASGSAGITGTMSQDTEVDMKEVELNELEPEKQPMNAASGAAMSLAGAEKNGLVKIKVA EDEAEAAAAAKFTGLSKEELLKVAGSPGWVRTRWALLLLFWLGWLGMLAGAVVIIVRAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAG NLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATK VKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLV TQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTV KGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLST |
Protein Functional Features |
| Main function of this protein. (from UniProt) |
| SLC3A2 (go to UniProt):P08195 |
| Retention analysis result of protein across 39 protein features of UniProt such as six molecule processing features, 13 region features, four site features, six amino acid modification features, two natural variation features, five experimental info features, and 3 secondary structure features. Here, because of limited space for viewing, we only show the protein feature retention information belong to the 13 regional features. All retention annotation result can be downloaded at * Minus value of BPloci means that the break pointn is located before the CDS. |
| - Retained protein feature among the 13 regional features. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| P08195 | Region | 15 | 39 | Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite | Type=Deletion;Start=1;End=101 |
| P08195 | Region | 15 | 39 | Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite | Type=Deletion;Start=38;End=99 |
Gene Isoform Structures and Expression Levels for SLC3A2 |
| Gene structures of our canonical and alternative spliced genes of SLC3A2 * Click on the image to open the UCSC genome browser with custom track showing this image in a new window. |
| Expression levels of gene isoforms across GTEx. |
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| Expression levels of gene isoforms across TCGA. |
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Protein Structures |
| PDB and CIF files of the predicted protein structures * Here we show the 3D structure of the proteins using Mol*. AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Model confidence is shown from the pLDDT values per residue. pLDDT corresponds to the model’s prediction of its score on the local Distance Difference Test. It is a measure of local accuracy (from AlphfaFold website). To color code individual residues, we transformed individual PDB files into CIF format. |
| 3D view using mol* of P08195-1 |
| 3D view using mol* of P08195-2 |
| 3D view using mol* of P08195-3 |
| 3D view using mol* of P08195-4 |
pLDDT Score Distribution |
| pLDDT score distribution of the predicted protein structures from AlphaFold2 * AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. |
| pLDDT distribution across the protein length of P08195-1 |
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| pLDDT distribution across the protein length of P08195-2 |
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| pLDDT distribution across the protein length of P08195-3 |
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| pLDDT distribution across the protein length of P08195-4 |
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Ramachandran Plot of Protein Structures |
| Ramachandran plot of the torsional angles - phi (φ)and psi (ψ) - of the residues (amino acids) contained in this protein peptide. |
| Ramachandran plot of P08195-1 |
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| Ramachandran plot of P08195-2 |
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| Ramachandran plot of P08195-4 |
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Potential Active Site Information |
| The potential binding sites of these proteins were identified using SiteMap, a module of the Schrodinger suite. |
| UniProt-id | Site score | Size | D score | Volume | Exposure | Enclosure | Contact | Phobic | Philic | Balance | Don/Acc | Residues |
| P08195-1 | 1.047 | 116 | 0.964 | 364.266 | 0.564 | 0.768 | 1.017 | 0.29 | 1.339 | 0.217 | 0.849 | 226,227,228,229,230,231,260,262,263,264,284,307,31 2,313,314,315,316,317,346,348,349,381,403,436,437, 438,439,440,443,444,467,468,484,485,486,487 |
| P08195-2 | 1.038 | 92 | 0.907 | 320.362 | 0.516 | 0.799 | 1.119 | 0.144 | 1.454 | 0.099 | 0.855 | 125,126,127,128,129,130,159,161,162,163,183,206,21 1,212,216,245,247,337,338,339,342,366,367,374,375, 383,384,385,386 |
| P08195-3 | 1.036 | 119 | 0.976 | 392.735 | 0.556 | 0.751 | 0.986 | 0.341 | 1.273 | 0.268 | 0.881 | 164,165,166,167,168,169,198,200,201,222,245,250,25 1,284,286,287,318,319,340,341,374,375,376,377,378, 381,382,405,406,422,423,424,425 |
| P08195-4 | 1.038 | 107 | 0.953 | 396.165 | 0.556 | 0.755 | 1.064 | 0.227 | 1.35 | 0.168 | 0.711 | 257,258,259,260,261,262,291,292,293,294,315,338,34 0,343,344,348,377,379,380,383,412,434,467,468,469, 470,471,474,475,498,499,515,516,517,518 |
Protein Structure and Feature Comparision |
| Protein Structure Comparision Using Template Modeling Scores (TM-score). |
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| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Canonical validated structure (PDB)(green) |
| 3D view using mol* of P08195-1_P08195-1_7ccs_A.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical validated structure (PDB)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of P08195-1_7ccs_A_P08195-2.pdb |
| 3D view using mol* of P08195-1_7ccs_A_P08195-3.pdb |
| 3D view using mol* of P08195-1_7ccs_A_P08195-4.pdb |
| Protein Structure Comparision Visualization with mol*. between Canonical predicted structure (AF2)(orange) vs Alternative predicted structure (AF2)(green) |
| 3D view using mol* of P08195-1_P08195-2.pdb |
| 3D view using mol* of P08195-1_P08195-3.pdb |
| 3D view using mol* of P08195-1_P08195-4.pdb |
| Protein Feature Comparison of the protein sequendary structures among the protiens. |
| ./stats/secondary_structure/figure/P08195-1_vs_P08195-2.png |
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| ./stats/secondary_structure/figure/P08195-1_vs_P08195-3.png |
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| ./stats/secondary_structure/figure/P08195-1_vs_P08195-4.png |
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| Protein Feature Comparison of the relative accessible surface area (ASA) among the protiens. |
| ./stats/relative_asa/P08195-1_vs_P08195-2.png |
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| ./stats/relative_asa/P08195-1_vs_P08195-3.png |
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| ./stats/relative_asa/P08195-1_vs_P08195-4.png |
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Protein-Protein Interaction |
| Interactors from UniProt. |
| Accession_id | Subsection | Start | End | Funcitonal feature | Splicing information |
| Interactors from STRING. |
| Gene name | Interactors |
Related Drugs to SLC3A2 |
| Drugs targeting this gene/protein. (DrugBank) |
| UniProt accession | Gene name | DrugBank ID | Drug name | Drug group | Actions |
Related Diseases to SLC3A2 |
| Previous studies relating to the alternative splicing of SLC3A2 and disease information from the MeSH term (PubMed) |
| Gene | PMID | Title | Abstract | MeSH ID | MeSH term |
| SLC3A2 | 11406111 | Human cystine/glutamate transporter: cDNA cloning and upregulation by oxidative stress in glioma cells. | A human cDNA for amino acid transport system x(C)(-) was isolated from diethyl maleate-treated human glioma U87 cells. U87 cells expressed two variants of system x(C)(-) transporters hxCTa and hxCTb with altered C-terminus regions probably generated by the alternative splicing at 3'-ends. Both hxCTa and hxCTb messages were also detected in spinal cord, brain and pancreas, although the level of hxCTb expression appears to be lower than that of hxCTa in these tissues. When expressed in Xenopus oocytes, hxCTb required the heavy chain of 4F2 cell surface antigen (4F2hc) and exhibited the Na(+)-independent transport of L-cystine and L-glutamate, consistent with the properties of system x(C)(-). In agreement with this, 137 kDa band was detected by either anti-xCT or anti-4F2hc antibodies in the non-reducing condition in western blots, whereas it shifted to 50 kDa or 90 kDa bands in the reducing condition, indicating the association of two proteins via disulfide bands. We found that the expression of xCT was rapidly induced in U87 cells upon oxidative stress by diethyl maleate treatment, which was accompanied by the increase in the L-cystine uptake by U87 cells. Because of this highly regulated nature, xCT in glial cells would fulfill the task to protect neurons against oxidative stress by providing suitable amount of cystine to produce glutathione. | D005910 | Glioma |
| SLC3A2 | 24711643 | Identifying biological pathways that underlie primordial short stature using network analysis. | Mutations in CUL7, OBSL1 and CCDC8, leading to disordered ubiquitination, cause one of the commonest primordial growth disorders, 3-M syndrome. This condition is associated with i) abnormal p53 function, ii) GH and/or IGF1 resistance, which may relate to failure to recycle signalling molecules, and iii) cellular IGF2 deficiency. However the exact molecular mechanisms that may link these abnormalities generating growth restriction remain undefined. In this study, we have used immunoprecipitation/mass spectrometry and transcriptomic studies to generate a 3-M 'interactome', to define key cellular pathways and biological functions associated with growth failure seen in 3-M. We identified 189 proteins which interacted with CUL7, OBSL1 and CCDC8, from which a network including 176 of these proteins was generated. To strengthen the association to 3-M syndrome, these proteins were compared with an inferred network generated from the genes that were differentially expressed in 3-M fibroblasts compared with controls. This resulted in a final 3-M network of 131 proteins, with the most significant biological pathway within the network being mRNA splicing/processing. We have shown using an exogenous insulin receptor (INSR) minigene system that alternative splicing of exon 11 is significantly changed in HEK293 cells with altered expression of CUL7, OBSL1 and CCDC8 and in 3-M fibroblasts. The net result is a reduction in the expression of the mitogenic INSR isoform in 3-M syndrome. From these preliminary data, we hypothesise that disordered ubiquitination could result in aberrant mRNA splicing in 3-M; however, further investigation is required to determine whether this contributes to growth failure. | D004392 | Dwarfism |
| SLC3A2 | 24711643 | Identifying biological pathways that underlie primordial short stature using network analysis. | Mutations in CUL7, OBSL1 and CCDC8, leading to disordered ubiquitination, cause one of the commonest primordial growth disorders, 3-M syndrome. This condition is associated with i) abnormal p53 function, ii) GH and/or IGF1 resistance, which may relate to failure to recycle signalling molecules, and iii) cellular IGF2 deficiency. However the exact molecular mechanisms that may link these abnormalities generating growth restriction remain undefined. In this study, we have used immunoprecipitation/mass spectrometry and transcriptomic studies to generate a 3-M 'interactome', to define key cellular pathways and biological functions associated with growth failure seen in 3-M. We identified 189 proteins which interacted with CUL7, OBSL1 and CCDC8, from which a network including 176 of these proteins was generated. To strengthen the association to 3-M syndrome, these proteins were compared with an inferred network generated from the genes that were differentially expressed in 3-M fibroblasts compared with controls. This resulted in a final 3-M network of 131 proteins, with the most significant biological pathway within the network being mRNA splicing/processing. We have shown using an exogenous insulin receptor (INSR) minigene system that alternative splicing of exon 11 is significantly changed in HEK293 cells with altered expression of CUL7, OBSL1 and CCDC8 and in 3-M fibroblasts. The net result is a reduction in the expression of the mitogenic INSR isoform in 3-M syndrome. From these preliminary data, we hypothesise that disordered ubiquitination could result in aberrant mRNA splicing in 3-M; however, further investigation is required to determine whether this contributes to growth failure. | D006130 | Growth Disorders |
| SLC3A2 | 24711643 | Identifying biological pathways that underlie primordial short stature using network analysis. | Mutations in CUL7, OBSL1 and CCDC8, leading to disordered ubiquitination, cause one of the commonest primordial growth disorders, 3-M syndrome. This condition is associated with i) abnormal p53 function, ii) GH and/or IGF1 resistance, which may relate to failure to recycle signalling molecules, and iii) cellular IGF2 deficiency. However the exact molecular mechanisms that may link these abnormalities generating growth restriction remain undefined. In this study, we have used immunoprecipitation/mass spectrometry and transcriptomic studies to generate a 3-M 'interactome', to define key cellular pathways and biological functions associated with growth failure seen in 3-M. We identified 189 proteins which interacted with CUL7, OBSL1 and CCDC8, from which a network including 176 of these proteins was generated. To strengthen the association to 3-M syndrome, these proteins were compared with an inferred network generated from the genes that were differentially expressed in 3-M fibroblasts compared with controls. This resulted in a final 3-M network of 131 proteins, with the most significant biological pathway within the network being mRNA splicing/processing. We have shown using an exogenous insulin receptor (INSR) minigene system that alternative splicing of exon 11 is significantly changed in HEK293 cells with altered expression of CUL7, OBSL1 and CCDC8 and in 3-M fibroblasts. The net result is a reduction in the expression of the mitogenic INSR isoform in 3-M syndrome. From these preliminary data, we hypothesise that disordered ubiquitination could result in aberrant mRNA splicing in 3-M; however, further investigation is required to determine whether this contributes to growth failure. | D009123 | Muscle Hypotonia |
Clinically important variants in SLC3A2 |
| (ClinVar, 04/20/2024) |
| accession_id | uniprot_id | gene_name | Type | Variant | Clinical_significance |
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