Navigation

1. Overview of ASpdb.
2. Understanding of ASpdb's Annotation Category.
- Gene Search Result Page.
- Gene Annotation Result Page.
-- 1) Protein Summary.
-- 2) AS Summary.
-- 3) Protein Functional Features.
-- 4) Gene Isoform Structures and Expression Levels
-- 5) Protein Structure.
-- 6) pLDDT Score Distribution.
-- 7) Ramachandran Plot of Protein Structure.
-- 8) Potential Active Site Information.
-- 9) Protein Structure and Feature Comparision.
-- 10) Protein-Protein Interaction.
-- 11) Related Drugs.
-- 12) Related Diseases.
-- 13) Clinically Important Variants.
12. Download Data and Contact Us.

1. Overview of ASpdb.
Alternative splicing is an important cellular process in eukaryotes, altering pre-mRNA to yield multiple protein isoforms from a single gene. To enhance our understanding of the impact of alternative splicing events on protein structures, we developed Aspdb, an expansive database that integrates experimentally determined structures with AlphaFold 2 (AF2) predicted models for human alternative splicing isoforms. Our database includes more than 3,400 canonical isoforms, each with both experimentally determined and AF2 predicted structures, alongside over 7,400 alternative isoforms for which we have generated AF2 predicted structures. ASpdb provides information on the alternative splicing events in human, including protein isoform 3D structure data, sequence variation, structural attributes, functional annotations, and comparative analyses of structural alterations among the isoforms, providing insights into their potential biological impacts. ASpdb emerges as a crucial resource for the scientific community, facilitating advances in the fields of alternative splicing, structural biology, and disease research.

2. Understanding ASpdb's Annotation Categories
Search page, example: BRCA1
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Input query
- Official HUGO gene symbol.

Gene Search Result Page
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Select your gene from the gene list.

Gene Annotation Result Page
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These are ASpdb's annotation categories for your query with links to their corresponding annotation parts.

1) Protein Summary.
This category shows the information of the protein.
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2) AS Summary.
This category shows the alternative splicing event information of your protein.
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3) Protein Functional Features.
This category provides the retention information of 39 protein features of individual isoforms compared to the canonical one.
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4) Gene Isoform Structures and Expression Levels.
This category provides the gene isoform structures and gene isoform expressions.
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5) Protein Structure.
This category visualize the 3D strucure of all isoform proteins.
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6) pLDDT Score Distribution.
This category visualize the reliability related information of the predicted protein structures like the pLDDT scores distribution across the protein length with the best active site residues marked with blue at the bottom.
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7) Ramachandran Plot of Protein Structure.
This category shows the distribution of torsion angles in the protein structures.
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8) Potential Active Site Information.
This table provides the detailed information of individual active sites of protein.
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9) Protein Structure and Feature Comparision
This table provides the structural and structure feature comparisons among all protein isoforms.
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10) Protein-Protein Interaction
This table provides the lost or retained interaction with other bioentities and validated PPI interactions.
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11) Related Drugs
This table provides the related drug information from DrugBank.
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12) Related Diseases
This table provides disease information from the PubMed article related to the alternative splicing and our protein.
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13) Clinically Important Variants
This category provides the ClinVar mutation information of our protein.
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12. Download data and contact us
Please go to download page and contact page.